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KMID : 0613820130230060825
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Journal of Life Science
2013 Volume.23 No. 6 p.825 ~ p.831
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Altered Gene Expression of Inflammatory Cytokines in Adipose Tissue of Streptozotocin-induced Diabetic C57BL/6 Mice
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Lee Yong-Ho
Kim Jong-Bong
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Abstract
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The aim of this study was to investigate the effects of induced diabetes by streptozotocin (STZ) administration on gene expression of proinflammatory cytokines in adipose tissue of C57/BL6 mice fed either a normal diet (ND) or a high-fat diet (HFD). Four diabetic mice groups (16- or 26-week-old mice fed either ND or HFD) and four control groups of age and diet matched non-diabetic mice were used. By real-time PCR, gene expression levels of tumor necrosis factor-α (TNF-α) and monocyte chemoattractant protein-1 (MCP-1) were examined in adipose tissue. The results demonstrated that gene expression of TNF-α was significantly or marginally increased in STZ induced diabetic mice groups compared with non-diabetic groups. On the other hand, MCP-1 gene expression tended to be decreased in diabetic mice compared with non-diabetic controls. Especially, MCP-1 expression level in 16w diabetic mice on HFD was about 26% of that in age and diet matched non-diabetic controls (p<0.001). In addition, MCP-1 gene expression in adipose tissue was correlated with plasma insulin levels (p=0.0002). These results suggest that gene expression of proinflammatory cytokines in adipose tissue is differentially regulated in mouse models of diabetes. The basic data in this study will be useful for elucidating basic mechanisms of inflammatory state and increased expression of proinflammatory cytokines in adipose tissue in obesity, insulin resistance, and diabetes.
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KEYWORD
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Adipose tissue, diabetes mellitus, insulin resistance, monocyte chemoattractant protein-1(MCP-1), tumor necrosis factor-α (TNF-α)
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